US 9,808,490 B2
Induced hepatocytes and uses thereof
Jau-Nan Lee, Kaohsiung (TW); Tony Tung-Yin Lee, Yakima, WA (US); Yuta Lee, Kaohsiung (TW); and Eing-Mei Tsai, Kaohsiung (TW)
Assigned to ACCELERATED BIOSCIENCES CORP., Carlsbad, CA (US)
Filed by Accelerated BioSciences Corp., Manhattan Beach, CA (US)
Filed on Nov. 25, 2015, as Appl. No. 14/952,023.
Claims priority of provisional application 62/085,185, filed on Nov. 26, 2014.
Prior Publication US 2016/0199416 A1, Jul. 14, 2016
Int. Cl. C12N 5/077 (2010.01); A61K 35/407 (2015.01); G01N 33/50 (2006.01); C12N 5/071 (2010.01); A61K 35/545 (2015.01)
CPC A61K 35/407 (2013.01) [C12N 5/067 (2013.01); C12N 5/0671 (2013.01); G01N 33/5023 (2013.01); G01N 33/5067 (2013.01); A61K 35/545 (2013.01); C12N 2501/115 (2013.01); C12N 2501/12 (2013.01); C12N 2501/155 (2013.01); C12N 2501/20 (2013.01); C12N 2501/237 (2013.01); C12N 2501/39 (2013.01); C12N 2501/40 (2013.01); C12N 2501/65 (2013.01); C12N 2506/025 (2013.01)] 26 Claims
 
1. A method of inducing a trophoblast stem (TS) cell to differentiate into an induced hepatocyte in vitro, comprising:
contacting the trophoblast stem cell with a fibroblast growth factor (FGF) that binds to FGFR1 to induce formation of a definitive endoderm (DE) cell, and
contacting the DE cell with a medium to induce differentiation into an induced hepatocyte,
wherein the medium comprises a FGF that binds to FGFR1, a glucocorticoid steroid, and an interleukin 6 group cytokine.