US 9,808,454 B2
Compositions for the mobilization, homing, expansion and differentiation of stem cells and methods of using the same
David Bar-Or, Englewood, CO (US); and Greg Thomas, Highlands Ranch, CO (US)
Assigned to Ampio Pharmaceuticals, Inc., Englewood, CO (US)
Filed by Ampio Pharmaceuticals, Inc., Greenwood Village, CO (US)
Filed on Mar. 17, 2014, as Appl. No. 14/216,430.
Claims priority of provisional application 61/791,623, filed on Mar. 15, 2013.
Claims priority of provisional application 61/832,713, filed on Jun. 7, 2013.
Claims priority of provisional application 61/897,449, filed on Oct. 30, 2013.
Claims priority of provisional application 61/923,314, filed on Jan. 3, 2014.
Claims priority of provisional application 61/939,625, filed on Feb. 13, 2014.
Prior Publication US 2014/0286913 A1, Sep. 25, 2014
Int. Cl. A01K 63/00 (2017.01); A61K 31/495 (2006.01); A61K 31/405 (2006.01); A61K 31/20 (2006.01); A61K 35/28 (2015.01); C12N 5/0775 (2010.01); A61K 35/32 (2015.01)
CPC A61K 31/495 (2013.01) [A61K 31/20 (2013.01); A61K 31/405 (2013.01); A61K 35/28 (2013.01); C12N 5/0663 (2013.01); A61K 35/32 (2013.01); C12N 2501/999 (2013.01)] 26 Claims
 
1. A method of expanding bone marrow derived mesenchymal stem cells, comprising
(a) contacting the mesenchymal stem cells with DA-DKP, wherein the DA-DKP is in a composition comprising a less than 5000 molecular weight fraction of human serum albumin, and
(b) culturing the stem cells under conditions suitable to facilitate the expansion of the mesenchymal stem cells.