US 9,808,408 B2
Biomarker-based methods for formulating compositions that improve skin quality and reduce the visible signs of aging in skin for individuals in a selected population
Qing Stella, Cincinnati, OH (US); Karl Shiqing Wei, Mason, OH (US); Cynthia Ann Garza, Cincinnati, OH (US); Kenneth Robert Wehmeyer, Cincinnati, OH (US); and Rohan Lalith Wimalasena, Mason, OH (US)
Assigned to The Procter & Gamble Company, Cincinnati, OH (US)
Filed by Qing Stella, Cincinnati, OH (US); Karl Shiqing Wei, Mason, OH (US); Cynthia Ann Garza, Cincinnati, OH (US); Kenneth Robert Wehmeyer, Cincinnati, OH (US); and Rohan Lalith Wimalasena, Mason, OH (US)
Filed on Jan. 16, 2011, as Appl. No. 13/7,632.
Claims priority of provisional application 61/295,732, filed on Jan. 17, 2010.
Claims priority of provisional application 61/295,826, filed on Jan. 18, 2010.
Prior Publication US 2011/0257030 A1, Oct. 20, 2011
This patent is subject to a terminal disclaimer.
Int. Cl. A61Q 19/10 (2006.01); A61K 8/31 (2006.01); A61K 8/03 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); G01N 33/92 (2006.01); G06Q 30/02 (2012.01)
CPC A61K 8/31 (2013.01) [A61K 8/03 (2013.01); A61Q 19/10 (2013.01); G01N 33/5088 (2013.01); G01N 33/6812 (2013.01); G01N 33/6827 (2013.01); G01N 33/6869 (2013.01); G01N 33/6881 (2013.01); G01N 33/92 (2013.01); G06Q 30/0201 (2013.01); A61K 2800/87 (2013.01); G01N 2333/4742 (2013.01); G01N 2333/545 (2013.01); G01N 2500/04 (2013.01)] 6 Claims
 
1. A method of identifying a rinse off personal care composition as effective for improving the stratum corneum barrier in a human subject who is a member of a selected population of subjects, comprising:
(a) generating one or more control skin profiles for two or more subjects in a select population of subjects wherein the subjects in the select population have dry or damaged skin;
(b) contacting at least a portion of skin of the subjects with a rinse-off test composition, rinsing the test composition off the portion of skin, extracting one or more skin samples from each of the subjects, and generating from the extracted samples one or more test profiles for the subjects;
(c) comparing the one or more test profiles to the one or more control profiles and identifying the rinse-off test composition as effective for improving the stratum corneum barrier in a human subject who is a member of a selected population of subjects if the test profile is directionally shifted away from the control profile and shows (i) a decrease in one or more inflammatory cytokines, (ii) an increase in one or more natural moisturizing factors, (iii) an increase in one or more lipids, and (iv) a decrease in total protein.