US 9,808,336 B2
Two stage cellularization strategy for the fabrication of bioartificial hearts
Ravi K. Birla, Sugar Land, TX (US)
Assigned to UNIVERSITY OF HOUSTON SYSTEM, Houston, TX (US)
Filed by Ravi K. Birla, Sugar Land, TX (US)
Filed on May 1, 2015, as Appl. No. 14/701,930.
Claims priority of provisional application 61/987,962, filed on May 2, 2014.
Prior Publication US 2015/0335417 A1, Nov. 26, 2015
Int. Cl. A61F 2/02 (2006.01); A61F 2/08 (2006.01); A61M 1/12 (2006.01); C12N 5/077 (2010.01); A61L 27/22 (2006.01); A61L 27/34 (2006.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01)
CPC A61F 2/08 (2013.01) [A61L 27/225 (2013.01); A61L 27/34 (2013.01); A61L 27/3604 (2013.01); A61L 27/3826 (2013.01); A61L 27/3834 (2013.01); A61M 1/12 (2013.01); A61M 1/122 (2014.02); C12N 5/0657 (2013.01); A61L 2430/20 (2013.01); A61L 2430/40 (2013.01); C12N 2533/56 (2013.01); C12N 2533/90 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method of fabricating a bioartificial heart comprising:
obtaining and/or isolating cells from a subject;
preparing a scaffold;
transplanting the cells within the scaffold by direct cell transplant,
wherein the direct cell transplant comprises delivering the cells to the scaffold by a plurality of direct injections; and
culturing the scaffold transplanted with the cells in a perfusion culture apparatus, wherein the perfusion culture apparatus mimics in vivo conditions.