US 9,807,986 B2
Methods for establishing animal model of hepatocellular carcinoma bone metastasis
Rui Hou, Wuhan (CN); Yuwei Wang, Wuhan (CN); Huifang Liang, Wuhan (CN); Zhanguo Zhang, Wuhan (CN); Zhimin Liu, Wuhan (CN); Binhao Zhang, Wuhan (CN); Bixiang Zhang, Wuhan (CN); and Xiaoping Chen, Wuhan (CN)
Filed by Rui Hou, Wuhan (CN); Yuwei Wang, Wuhan (CN); Huifang Liang, Wuhan (CN); Zhanguo Zhang, Wuhan (CN); Zhimin Liu, Wuhan (CN); Binhao Zhang, Wuhan (CN); Bixiang Zhang, Wuhan (CN); and Xiaoping Chen, Wuhan (CN)
Filed on Mar. 28, 2016, as Appl. No. 15/83,269.
Prior Publication US 2017/0273283 A1, Sep. 28, 2017
Int. Cl. A01K 67/027 (2006.01); A61K 49/00 (2006.01); G01N 33/50 (2006.01); C12N 5/09 (2010.01); C12N 9/02 (2006.01)
CPC A01K 67/0271 (2013.01) [C12N 5/0693 (2013.01); C12N 9/0069 (2013.01); C12Y 113/12007 (2013.01); A01K 2207/12 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0331 (2013.01)] 3 Claims
 
1. A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method comprising:
1) transfecting 97H and LM3 cell clones with luciferase genes to yield 97H/Luc+ and LM3/Luc+ cells expressing firefly luciferase (LUC);
2) intracardially injecting the 97H/Luc+ and LM3/Luc+ cells into each nude mouse of a set of nude mice to induce the formation of metastatic 97H/Luc+ and LM3/Luc+ cells in bones of the set of nude mice, wherein the nude mice are 4-5-week-old BALB/cA-nu mice; and
3) isolating the metastatic 97H/Luc+ and LM3/Luc+ cells obtained in 2) from the bones of the set of nude mice.