US 7,468,429 B2
	Recombinant Candida rugosa lipases
Jei-Fu Shaw, Taipei (Taiwan); Guan-Chiun Lee, Taipei (Taiwan); and Shye-Jye Tang, Taipei (Taiwan)
Assigned to Academia Sinica, Taipei (Taiwan)
Filed on Feb. 07, 2006, as Appl. No. 11/349,592.
Application 11/349592 is a division of application No. 09/943857, filed on Aug. 31, 2001, granted, now 7,052,879.
Prior Publication US 2006/0121528 A1, Jun. 08, 2006
Int. Cl. C07H 21/04 (2006.01); C12N 9/16 (2006.01); C12P 21/00 (2006.01)

U.S. Cl. 536—23.1  [435/196; 435/71.1]

11 Claims

1. An isolated nucleic acid comprising a DNA that encodes, in a non-Candida rugosa cell, a lipase at least 98% identical to SEQ ID NO:2, wherein the DNA has at least 15 CTG codons encoding the serines in SEQ ID NO:2, when expressed in Candida rugosa, replaced with a universal serine codon selected from TCT, TCC, TCA, TCG, AGT, or AGC.