| US 7,468,273 B2 | ||
| Canine GHRH gene, polypeptides and methods of use | ||
| Laurent Bernard Fisher, Sainte Foy les Lyon (France); Nathalie Michele Cachet, Sainte Foy les Lyon (France); and Simona Barzu-Le-Roux, Lentilly (France) | ||
| Assigned to Meial Limited, Duluth, Ga. (US) | ||
| Filed on Dec. 17, 2004, as Appl. No. 11/15,461. | ||
| Application 11/015461 is a continuation in part of application No. 10/838122, filed on May 03, 2004. | ||
| Claims priority of provisional application 60/467405, filed on May 01, 2003. | ||
| Prior Publication US 2005/0164946 A1, Jul. 28, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 15/63 (2006.01); A61K 48/00 (2006.01) | ||
| U.S. Cl. 435—320.1 [514/44] | 20 Claims |
| 1. An expression vector for expressing a canine pro-GHRH deleted of the propeptide N-terminus comprising a polynucleotide
operatively linked to a promoter and optionally to an enhancer, wherein the polynucleotide comprises
(a) a polynucleotide encoding a canine proGHRH N-terminus deleted polypeptide having at least 95% sequence identity to amino
acids 31 to 106 of SEQ ID NO: 1; or
(b) a polynucleotide having at least 95% sequence identity to nucleotides 91 to 321 of SEQ ID NO:3 and encoding a canine proGHRH
N-terminus deleted polypeptide; and
(c) a polynucleotide encoding a secretory signal peptide sequence fused to the nucleotide base sequence from nucleotide 91
to nucleotide 321 of SEQ ID NO: 3;
wherein the canine pro-GHRH polypeptide deleted of the propeptide N-terminus, when expressed in vivo in a canine is able to
be cellularly processed to mature canine GHRH with the ability to stimulate growth hormone secretion.
|