| US 7,468,257 B2 | ||
| Protein S functional assay | ||
| Yong Dai, Mechanicsville, Va. (US); Biqing Ye, Bergenfield, N.J. (US); Kui Chen, Raleigh, N.C. (US); Pau Bruguera, Barcelona (Spain); Daniel E Lawson, Wakeforest, N.C. (US); and Shamay Tang, Miami, Fla. (US) | ||
| Assigned to Instrumentation Laboratory Corporation, Lexington, Mass. (US) | ||
| Filed on Dec. 15, 2004, as Appl. No. 11/13,121. | ||
| Application 11/013121 is a continuation of application No. 10/029406, filed on Dec. 19, 2001, granted, now 6,855,509. | ||
| Claims priority of provisional application 60/256703, filed on Dec. 19, 2000. | ||
| Prior Publication US 2005/0095662 A1, May 05, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12Q 1/37 (2006.01); G01N 33/86 (2006.01) | ||
| U.S. Cl. 435—23 [436/69] | 15 Claims |
| 1. A method for measuring protein S activity in a plasma sample, comprising the steps of:
(a) mixing a sample of test plasma with protein S deficient plasma,
at least one recombinant tissue factor selected from the group consisting of recombinant rabbit, recombinant equine, recombinant
porcine, and recombinant human tissue factors,
purified or synthetic phospholipids comprising phosphocholine, phosphoserine, and phosphoethanolamine,
an activator of protein C,
calcium ion,
(b) measuring a clotting time of the sample; and
(c) comparing the measurement in (b) to a standard curve derived from clotting times of plasma samples having a range of known
protein S activities.
|