| US 7,468,254 B2 | ||
| Cytotoxicity mediation of cells evidencing surface expression of MCSP | ||
| David S. F. Young, Toronto (Canada); Susan E. Hahn, Toronto (Canada); Helen P. Findlay, Toronto (Canada); and Alison L. Ferry, Toronto (Canada) | ||
| Assigned to Arius Research Inc., Toronto, Ontario (Canada) | ||
| Filed on Sep. 24, 2004, as Appl. No. 10/949,846. | ||
| Application 10/949846 is a continuation in part of application No. 10/810744, filed on Mar. 26, 2004, granted, now 7,393,531. | ||
| Application 10/810744 is a continuation in part of application No. 10/762129, filed on Jan. 20, 2004, granted, now 7,361,342. | ||
| Application 10/762129 is a continuation in part of application No. 10/743451, filed on Dec. 19, 2003, abandoned. | ||
| Application 10/743451 is a continuation of application No. 10/348231, filed on Jan. 21, 2003, granted, now 7,009,040, filed on Mar. 07, 2006. | ||
| Prior Publication US 2005/0063967 A1, Mar. 24, 2005 | ||
| Int. Cl. G01N 33/53 (2006.01); G01N 33/574 (2006.01); C07K 16/00 (2006.01) | ||
| U.S. Cl. 435—7.1 [435/7.23; 530/387.1; 530/388.1; 530/388.8] | 4 Claims |
| 1. A binding essay to determine a presence of cells which express the melanoma-associated chondroitin sulfate proteoglycan
epitope which specifically binds to the isolated monoclonal antibody produced from the hybridome deposited with the ATCC as
PTA-5643, or an antigen binding fragment thereof comprising:
providing a cell sample;
providing an isolated monoclonal antibody of antigen binding fragment thereof which binds to the same melanoma-associated
chondroitin sulfate proteoglycan epitope as the monoclonal antibody produced from said hybridoma;
contacting said isolated monoclonal antibody of antigen binding fragment thereof with said cell sample; and
determining binding of said isolated monoclonal antibody or antigen binding fragment thereof with said cell sample;
whereby the presence of cells which express said melanoma-associated chondroitin sulfate proteoglycan epitope is determined.
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