| US 7,468,192 B2 | ||
| Method for repair of cartilage lesions | ||
| Shuichi Mizuno, Brookline, Mass. (US); Akihiko Kusanagi, Brookline, Mass. (US); Laurence J. B. Tarrant, Easthampton, Mass. (US); Toshimasa Tokuno, Tokyo (Japan); and Robert Lane Smith, Palo Alto, Calif. (US) | ||
| Assigned to Histogenics Corporation, Waltham, Mass. (US) | ||
| Filed on Jul. 22, 2003, as Appl. No. 10/625,822. | ||
| Application 10/625822 is a continuation in part of application No. 10/104677, filed on Mar. 22, 2002, granted, now 6,949,252. | ||
| Claims priority of provisional application 60/427627, filed on Nov. 18, 2002. | ||
| Claims priority of provisional application 60/425696, filed on Nov. 12, 2002. | ||
| Prior Publication US 2004/0134502 A1, Jul. 15, 2004 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A61F 2/00 (2006.01); C12N 11/02 (2006.01); C12N 11/08 (2006.01); C12N 5/06 (2006.01); C12N 5/08 (2006.01) | ||
| U.S. Cl. 424—423 [424/93.7; 435/177; 435/180; 435/395] | 13 Claims |
| 1. A method for repair of cartilage lesions, said method comprising steps:
a) preparing a porous support matrix containing a plurality of pores having a pore size from about 50 to about 500 μm, wherein
said matrix is prepared as a porous sponge, porous scaffold, porous honeycomb or porous honeycomb-like lattice;
b) seeding said support matrix with chondrocytes isolated from a donor and suspended in a collagenous solution or collagenous
gel;
c) applying a constant or cyclic hydrostatic pressure to the support matrix seeded with chondrocytes from step (b) for a period
from about one hour to about 30 days followed by a resting period at a static atmospheric pressure for about one day to about
60 days,
wherein said hydrostatic pressure is from about 0.01 MPa to about 10 MPa above atmospheric pressure;
d) pre-treating a cartilage lesion by depositing a layer of a biologically acceptable bottom sealant into said lesion before
implanting said seeded support matrix from step c) therein;
e) implanting said seeded support matrix from step c) into said pre-treated lesion of step d); and
f) depositing a layer of a biologically acceptable top sealant over said seeded support matrix from step c) implanted into
said lesion.
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