a broadband light source where a fluorescent spectrum is obtained from an excitation frequency provided by the light source;

a fluidic circuit illuminated by the light source having a plurality of flow channels defined therein through which at least one analyte flows;

a proximity detector array disposed below and aligned with the fluidic circuit for detecting light intensity from the light source transmitted through the fluidic circuit, including through the flow channels in which the at least one analyte flows;

a variable filter disposed between the detector array and the fluidic circuit so that each position of the detector array is provided with a different wavelength response thereby providing a hyper-spectral imaging array;

a blocking filter to reduce the excitation frequency from the detector array, while permitting transmission of an emission frequency,

wherein the blocking filter is characterized by a varying spectral position of the reflectivity edge; and

a processor to geometrically normalize the light to the filter characteristics and to spectrally normalize the light source during data acquisition, and to compare each specific wavelength/area under test to a specific solvent reference flow channel in the fluidic circuit.