| US 7,465,550 B2 | ||
| Method for screening taste-modulating compounds | ||
| Charles S. Zuker, San Diego, Calif. (US); Jon Elliot Adler, Sherwood, Oreg. (US); Nicholas J.P. Ryba, Bethesda, Md. (US); Ken Mueller, San Diego, Calif. (US); and Mark Hoon, Kensington, Md. (US) | ||
| Assigned to The Regents of the University of California, Oakland, Calif. (US); and The United States of America as represented by the Department of Health and Human Services, Washington, D.C. (US) | ||
| Filed on Oct. 07, 2004, as Appl. No. 10/962,365. | ||
| Application 10/962365 is a continuation of application No. 09/510332, filed on Feb. 22, 2000, granted, now 7,244,584. | ||
| Application 09/510332 is a continuation in part of application No. 09/393634, filed on Sep. 10, 1999, granted, now 6,558,910. | ||
| Prior Publication US 2005/0048586 A1, Mar. 03, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. G01N 33/53 (2006.01); G01N 33/567 (2006.01) | ||
| U.S. Cl. 435—7.1 [435/7.2] | 23 Claims |
| 1. A method for identifying a compound that modulates taste signaling in taste cells, the method comprising the steps of:
(i) contacting a taste transduction G protein-coupled receptor polypeptide with the compound, the polypeptide comprising greater
than 90% amino acid sequence identity to SEQ ID NO:7 and binding a bitter tastant; and
(ii) determining the functional effect of the compound upon the polypeptide, thereby identifying a compound that modulates
taste signaling in taste cells.
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