| US 7,462,464 B1 | ||
| Method for the detection of antimicrobial residues in food and bodily fluid samples | ||
| Pieter Cornelis Langeveld, Delft (Netherlands); Jacobus Stark, Rotterdam (Netherlands); and Petrus Andreas Van Paridon, Voorburg (Netherlands) | ||
| Assigned to DSM IP Assets B.V., (Netherlands) | ||
| Appl. No. 10/89,874 PCT Filed Oct. 03, 2000, PCT No. PCT/EP00/09874 § 371(c)(1), (2), (4) Date Sep. 03, 2002, PCT Pub. No. WO01/25471, PCT Pub. Date Apr. 12, 2001. |
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| Claims priority of application No. 99203264 (EP), filed on Oct. 04, 1999; and application No. 99203429 (EP), filed on Oct. 18, 1999. | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12Q 1/18 (2006.01); C12Q 1/02 (2006.01); C12N 1/20 (2006.01) | ||
| U.S. Cl. 435—32 [435/29; 435/252.5; 435/253.4] | 16 Claims |
| 1. A process for determining the presence or absence of an antimicrobial residue in a sample, which process comprises:
(i) contacting the sample with a test suitable for determining the presence or absence of an antimicrobial residue in the
sample;
(ii) inactivating any natural disturbing compound present in the sample which is capable of inhibiting the test and which
leads to a false positive result absent said inactivating step, by heating said contacted sample and test to a temperature
of 75° C. to 85° C. for a sufficient time interval to inactivate the natural disturbing compound that may be present in the
sample; followed by
(iii) incubating the contacted sample and test to determine whether microbial growth occurs, whereby the absence of microbial
growth indicates the presence of at least one antimicrobial residue.
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