US 11,835,460 B2
System and method with fiducials having offset layouts
John S. Vieceli, Encinitas, CA (US); Alex Nemiroski, San Diego, CA (US); Paul Belitz, San Diego, CA (US); Robert Langlois, San Diego, CA (US); M. Shane Bowen, Encinitas, CA (US); Danny Yuan Chan, San Diego, CA (US); Bala Murali K. Venkatesan, San Francisco, CA (US); Hui Han, San Diego, CA (US); Kevan Samiee, San Diego, CA (US); and Stephen Tanner, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Appl. No. 16/482,638
Filed by Illumina, Inc., San Diego, CA (US)
PCT Filed Jan. 31, 2018, PCT No. PCT/US2018/016189
§ 371(c)(1), (2) Date Jul. 31, 2019,
PCT Pub. No. WO2018/144574, PCT Pub. Date Aug. 9, 2018.
Claims priority of application No. 1701686 (GB), filed on Feb. 1, 2017.
Prior Publication US 2020/0011800 A1, Jan. 9, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/64 (2006.01); B01J 19/00 (2006.01); C12Q 1/6837 (2018.01)
CPC G01N 21/6456 (2013.01) [B01J 19/0046 (2013.01); C12Q 1/6837 (2013.01); G01N 21/6452 (2013.01); B01J 2219/0056 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00529 (2013.01); B01J 2219/00547 (2013.01); B01J 2219/00549 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00608 (2013.01); B01J 2219/00648 (2013.01); B01J 2219/00722 (2013.01); B01L 2300/021 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); H01J 2237/24578 (2013.01)] 13 Claims
OG exemplary drawing
 
1. An array, comprising:
a support including a swath where reaction sites are located, each reaction site including an oligonucleotide probe that is to hybridize to a biological sample, the biological sample comprising a nucleic acid sequence that responds in successive cycles of fluorescent imaging; and
six fiducial features formed in a hexagonal pattern on the support in the swath to define an image area, wherein each of the six fiducial features in the hexagonal pattern is a uniform distance from a center point of the image area, wherein each of the six fiducial features has a fluorescent material in a single circle shape that separates an inner area where some of the reaction sites are located and an outer area where some other of the reaction sites are located, wherein the fluorescent material responds in the successive cycles of fluorescent imaging, wherein the six fiducial features are disposed in rows that are offset with respect to one another, wherein in the offset rows, fiducials of a first row do not align with fiducials of an adjacent second row, relative to a line perpendicular to the first row and the adjacent second row, and wherein the some of the reaction sites in the outer area are positioned within the hexagonal pattern.