	US 7,460,961 B2
	N-TRADD active site and uses thereof
Desiree H. H. Tsao, Belmont, Mass. (US); Jean-Baptiste Telliez, Waltham, Mass. (US); Thomas McDonagh, Acton, Mass. (US); Lih-Ling Lin, Concord, Mass. (US); Sang Hsu, Lexington, Mass. (US); Guang-Yi Xu, Medford, Mass. (US); and A. Karl Malakian, Boxborough, Mass. (US)
Assigned to Genetics Institute, LLC, Cambridge, Mass. (US)
Filed on Jul. 20, 2004, as Appl. No. 10/894,834.
Application 10/894834 is a division of application No. 09/821819, filed on Mar. 29, 2001, abandoned.
Claims priority of provisional application 60/195370, filed on Apr. 06, 2000.
Prior Publication US 2005/0002898 A1, Jan. 06, 2005
Int. Cl. G06F 17/11 (2006.01); G06F 17/50 (2006.01); G06F 19/00 (2006.01)

U.S. Cl. 702—27 [702/19; 702/23]

47 Claims

1. A method for identifying an agent that interacts with N-terminal domain of TNFR- 1 associated death domain protein (N-TRADD), the method comprising:

providing a solution comprising N-TRADD;

displaying a three-dimensional model of an active site of N-TRADD, wherein the active site of N-TRADD comprises an N-terminal domain of TRADD that interacts with C-TRAF2 and wherein the three dimensional structure of the active site comprises the relative structural coordinates of amino acid residues Y16, L17, F18, V58, L59, K63, I64, H65, D68, Q70, I72, V73, Q74, L75, C78, L118, G121, A122, R124, L125, D149, E150, and L152 according to FIG. 2, ± a root mean square deviation from the conserved backbone atoms of the amino acids of the active site of not more than 1.5Å;

using the three-dimensional structure of the active site of N-TRADD to design an agent that interacts with the active site of N-TRADD; and

obtaining or synthesizing the agent.