| US 7,459,290 B1 | ||
| Methods of using functional 30S subunits | ||
| Gloria M. Culver, Ames, Iowa (US); and Jennifer A. Maki, Hermantown, Minn. (US) | ||
| Assigned to Iowa State University Research Foundation, Inc., Ames, Iowa (US) | ||
| Filed on Mar. 30, 2005, as Appl. No. 11/94,770. | ||
| Claims priority of provisional application 60/557606, filed on Mar. 30, 2004. | ||
| Claims priority of provisional application 60/640361, filed on Dec. 30, 2004. | ||
| Int. Cl. C12P 21/06 (2006.01); C07H 21/04 (2006.01); C07H 21/02 (2006.01) | ||
| U.S. Cl. 435—69.1 [536/23.1; 530/300; 530/350] | 22 Claims |
| 1. A method to prepare a peptide, comprising:
a) providing a defined translation reaction mixture comprising isolated i) 30S subunits comprising small subunit ribosomal
proteins and 16S rRNA, ii) 50S subunits comprising large subunit ribosomal proteins, 5S rRNA and 23S rRNA, iii) charged tRNAs
for at least one amino acid, iv) RNA template for translation with at least one codon corresponding to the anticodon in the
charged tRNAs, and v) one or more translation factors; and
b) incubating the mixture so as to yield a peptide encoded by the RNA template.
|