US 7,456,337 B2
Signal transduction stress-related proteins and methods of use in plants
Oswaldo da Costa e Silva, Apex, N.C. (US); Hans J. Bohnert, Tucson, Ariz. (US); Nocha van Thielen, Cary, N.C. (US); Ruoying Chen, Apex, N.C. (US); and Manabu Ishitani, Cary, N.C. (US)
Assigned to BASF Plant Science GmbH, (Germany)
Filed on Dec. 20, 2007, as Appl. No. 11/961,112.
Application 11/961112 is a division of application No. 09/828447, filed on Apr. 06, 2001, granted, now 6,720,477.
Claims priority of provisional application 60/196001, filed on Apr. 07, 2000.
Prior Publication US 2008/0168577 A1, Jul. 10, 2008
Int. Cl. C12N 15/82 (2006.01); C12N 15/29 (2006.01); A01H 5/00 (2006.01); A01H 5/10 (2006.01)
U.S. Cl. 800—289  [800/298; 800/320.1; 800/312; 800/306; 800/314; 800/320; 800/320.2; 800/320.3; 800/322; 800/317; 800/317.1; 800/317.2; 800/317.3; 800/317.4; 536/23.6; 435/419] 22 Claims
 
20. A method of producing a drought-tolerant transgenic plant, the method comprising the steps of:
a) transforming a plant cell with an expression cassette comprising a polynucleotide selected from the group consisting of:
i) a polynucleotide having a sequence comprising nucleotides 1 to 1800 of SEQ ID NO:10; and
ii) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 337 of SEQ ID NO:15;
b) growing the transformed plant cell to generate transgenic plants; and
c) screening the transgenic plants generated in step b) to identify a transgenic plant that expresses the polypeptide and exhibits increased tolerance to drought stress as compared to a wild type variety of the plant.