CPC G16B 40/00 (2019.02) [C12Q 1/37 (2013.01); C12Q 1/40 (2013.01); G16B 5/00 (2019.02); G01N 2333/928 (2013.01); G01N 2333/96416 (2013.01); G01N 2333/976 (2013.01)] | 16 Claims |
1. A method for rapidly estimating metabolizable energy of a goose diet, comprising the following steps:
(1) measuring and calculating, for the diet, a value of simulative digestion gross energy (SDGE) at one or more values of crude fiber wherein measuring and calculating the value of simulative digestion gross energy comprises simulated digestion in a gastric phase and simulated digestion in an intestinal phase, comprising the following steps:
preparing a simulated gastric fluid, a simulated gastric phase buffer, a simulated intestinal fluid, and a simulated intestinal phase buffer;
digesting the diet in the simulated gastric phase buffer containing the simulated gastric fluid and in simulated intestinal phase buffer containing the simulated intestinal fluid in sequence in a monogastric animal simulative digestion device;
collecting and weighing a residue after digestion, and calculating the value of simulative digestion gross energy is according to the following formula D:
wherein the SDGE is expressed in MJ/kg
wherein during the digestion:
a digestive enzyme in the simulated gastric fluid is pepsin, and the concentration of the pepsin 1475 U/ml;
a digestive enzyme in the simulated intestinal fluid is selected from trypsin, chymotrypsin, and amylase;
a digestion temperature both in a gastric phase and in an intestinal phase is in a range of 40.5-41.5° C.; and a digestion time in the gastric phase is 4-5 hours, and a digestion time in the intestinal phase is in a range of 12-16 hours; and
(2) calculating a rectified value of simulative digestion gross energy of the crude fiber in the diet by putting a crude fiber level in the diet to be measured into formula E for rectifying the value of simulative digestion gross energy; wherein formula E comprises:
Rectified value of simulative digestion of gross energy=−0.020*CF20.333*CF−0.476+SDGE
wherein CF represents value of crude fiber level, the unit of the rectified value of simulative digestion gross energy is MJ/kg, and SDGE represents the value of simulative digestion gross energy; and wherein the rectified value of simulative digestion gross energy mapped to the metabolizable energy of the goose diet and the rectified value of simulative digestion gross energy used as a value proximate to the metabolizable energy of the goose diet.
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