US 11,815,510 B2
Method for detecting a dengue infection
Kieran O'Donoghue, Dundalk (IE); and Rory Sobolewski, Laytown (IE)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Filed on Dec. 19, 2022, as Appl. No. 18/068,510.
Application 18/068,510 is a division of application No. 16/297,531, filed on Mar. 8, 2019, granted, now 11,561,218.
Claims priority of application No. 18160915 (EP), filed on Mar. 9, 2018.
Prior Publication US 2023/0117054 A1, Apr. 20, 2023
Int. Cl. G01N 33/50 (2006.01); G01N 15/14 (2006.01); G01N 15/02 (2006.01); G01N 15/06 (2006.01); G01N 15/00 (2006.01)
CPC G01N 33/5094 (2013.01) [G01N 15/0205 (2013.01); G01N 15/06 (2013.01); G01N 15/14 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/0084 (2013.01); G01N 2015/0693 (2013.01); G01N 2800/26 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for detecting a dengue infection in a patient blood sample, comprising the steps of:
a) performing a valid perox cells analysis comprising determining a number of valid perox cells detected passing through a flowcell from a perox channel;
b) performing a red blood cells analysis comprising determining a red blood cell count;
c) performing a platelet distribution analysis in the sample comprising determining the platelet distribution, a standard deviation of the platelet distribution, and a percentage mean of the platelet distribution, and determining a platelet distribution width by dividing the standard deviation by the percentage mean;
d) performing a neutrophil cluster analysis comprising determining a located mean of the neutrophil cluster on an x-axis in a scatter diagram and determining a located mean of the neutrophil cluster on a y-axis in the scatter diagram;
e) performing a raw red cell analysis comprising determining a raw red cell count on a Red Blood Cell (RBC) channel;
f) determining the percentage of Red Blood Cells (RBCs) with volumes less than 60 fL;
g) obtaining sample parameters from values determined in steps a)-f); and
h) evaluating the sample parameters in relation to a prespecified criterion, wherein, if the prespecified criterion is fulfilled, a dengue infection is present.