CPC G01N 33/5094 (2013.01) [G01N 15/0205 (2013.01); G01N 15/06 (2013.01); G01N 15/14 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/0084 (2013.01); G01N 2015/0693 (2013.01); G01N 2800/26 (2013.01)] | 10 Claims |
1. A method for detecting a dengue infection in a patient blood sample, comprising the steps of:
a) performing a valid perox cells analysis comprising determining a number of valid perox cells detected passing through a flowcell from a perox channel;
b) performing a red blood cells analysis comprising determining a red blood cell count;
c) performing a platelet distribution analysis in the sample comprising determining the platelet distribution, a standard deviation of the platelet distribution, and a percentage mean of the platelet distribution, and determining a platelet distribution width by dividing the standard deviation by the percentage mean;
d) performing a neutrophil cluster analysis comprising determining a located mean of the neutrophil cluster on an x-axis in a scatter diagram and determining a located mean of the neutrophil cluster on a y-axis in the scatter diagram;
e) performing a raw red cell analysis comprising determining a raw red cell count on a Red Blood Cell (RBC) channel;
f) determining the percentage of Red Blood Cells (RBCs) with volumes less than 60 fL;
g) obtaining sample parameters from values determined in steps a)-f); and
h) evaluating the sample parameters in relation to a prespecified criterion, wherein, if the prespecified criterion is fulfilled, a dengue infection is present.
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