US 11,815,460 B2
Optical systems and methods for biological analysis
Kevin Maher, Woodside, CA (US); Mingsong Chen, Singapore (SG); Jacob K. Freudenthal, San Jose, CA (US); Kuan Moon Boo, Singapore (SG); Soo Yong Lau, Singapore (SG); David Fortescue, San Francisco, CA (US); Ming Shen, Singapore (SG); and Woon Liang Terence Soh, Singapore (SG)
Assigned to LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on May 26, 2022, as Appl. No. 17/825,222.
Application 15/643,626 is a division of application No. 14/348,878, granted, now 9,702,823, issued on Jul. 11, 2017, previously published as PCT/US2012/058107, filed on Sep. 28, 2012.
Application 17/825,222 is a continuation of application No. 15/643,626, filed on Jul. 7, 2017, abandoned.
Claims priority of provisional application 61/541,453, filed on Sep. 30, 2011.
Claims priority of provisional application 61/541,495, filed on Sep. 30, 2011.
Prior Publication US 2022/0412890 A1, Dec. 29, 2022
Int. Cl. G01N 21/64 (2006.01); G01N 37/00 (2006.01)
CPC G01N 21/6486 (2013.01) [G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); G01N 2021/6471 (2013.01); G01N 2021/6478 (2013.01); G01N 2201/062 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of analyzing a plurality of biological sample volumes, the method comprising:
emitting, along an excitation optical path toward a sample holder holding the plurality of biological sample volumes, electromagnetic radiation from a broadband LED, wherein the broadband LED emits the electromagnetic radiation across a range of wavelengths at a total output power of at least 5 watts and at a maximum intensity at a wavelength less than 600 nanometers and at an intensity less than 30 percent of the maximum intensity at a wavelength of 650 or 670 nanometers,
passing the electromagnetic radiation emitted from the broadband LED through a first lens along the excitation optical path, the first lens directing the electromagnetic radiation from the broadband LED to the sample holder;
receiving, by a sensor, an electromagnetic emission transmitted from the sample holder along an emission optical path; and
passing the electromagnetic emission through a second lens disposed along the emission optical path between the sample holder and the sensor.