US 11,814,678 B2
Universal short adapters for indexing of polynucleotide samples
Tatjana Singer, San Diego, CA (US); Ryan Kelley, San Diego, CA (US); Gordon Bean, San Diego, CA (US); and Eric Vermaas, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on May 4, 2021, as Appl. No. 17/307,973.
Application 17/307,973 is a continuation of application No. 15/973,210, filed on May 7, 2018, granted, now 11,028,436.
Claims priority of provisional application 62/524,390, filed on Jun. 23, 2017.
Claims priority of provisional application 62/503,272, filed on May 8, 2017.
Prior Publication US 2021/0262026 A1, Aug. 26, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); G16B 25/00 (2019.01); C12Q 1/6869 (2018.01); G16B 30/00 (2019.01); G16B 30/10 (2019.01); G16B 25/20 (2019.01)
CPC C12Q 1/6874 (2013.01) [C12Q 1/6869 (2013.01); G16B 25/00 (2019.02); G16B 30/00 (2019.02); G16B 30/10 (2019.02); G16B 25/20 (2019.02)] 20 Claims
OG exemplary drawing
 
1. A method for processing target nucleic acids derived from a plurality of samples, comprising:
(a) contacting a plurality of index polynucleotides with target nucleic acids derived from the plurality of samples to generate a plurality of index-target polynucleotides, wherein:
index polynucleotides contacted with target nucleic acids derived from each sample comprise an index sequence or a combination of index sequences that is uniquely associated with that sample,
the index sequence or the combination of index sequences is selected from a set of index sequences, and
the set of index sequences comprises a plurality of pairs of color-balanced index sequences, wherein any two bases at corresponding sequence positions of each pair of color-balanced index sequences include both (i) an adenine (A) base or a cytosine (C) base, and (ii) a guanine (G) base, a thymine (T) base, or a uracil (U) base.