US 11,814,672 B2
Complex of LNA probe and graphene oxide and nucleic acid detection method using same
Jieon Lee, Daejeon (KR); Woo-keun Kim, Daejeon (KR); Seokjoo Yoon, Daejeon (KR); and Bomi Shin, Daejeon (KR)
Assigned to Korea Research Institute of Chemical Technology, Daejeon (KR)
Filed by Korea Research Institute of Chemical Technology, Daejeon (KR)
Filed on Aug. 30, 2020, as Appl. No. 17/006,889.
Claims priority of application No. 10-2020-0047039 (KR), filed on Apr. 17, 2020.
Prior Publication US 2021/0324471 A1, Oct. 21, 2021
Int. Cl. C12Q 1/6813 (2018.01)
CPC C12Q 1/6813 (2013.01) [C12Q 2531/119 (2013.01); C12Q 2537/137 (2013.01); C12Q 2537/1373 (2013.01); C12Q 2600/178 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for detecting a target nucleic acid comprising:
(a) mixing a molecular beacon containing locked nucleic acid (LNA) and a sample containing the target nucleic acid to form a composition, wherein the molecular beacon containing LNA is a double-stranded nucleic acid complex comprising a first single strand comprising a toehold region complementary to the target nucleic acid and a fluorescent reagent, and a second single strand bound with graphene oxide, wherein the first single strand and the second single strand are complementary to each other at least in part;
(b) complementarily binding the toehold region of the first single strand to the target nucleic acid and displacing the second single strand through toehold-mediated strand displacement; and
(c) detecting a fluorescence signal of the fluorescent reagent, thereby detecting the target nucleic acid.