US 11,814,671 B2
System and method for leakage control in a particle capture system
Kalyan Handique, Ann Arbor, MI (US); John Stark, Ann Arbor, MI (US); Yadwinder Deol, Ann Arbor, MI (US); and Vishal Sharma, Ann Arbor, MI (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Feb. 8, 2021, as Appl. No. 17/170,576.
Application 17/170,576 is a continuation of application No. 16/821,644, filed on Mar. 17, 2020, granted, now 10,947,581.
Application 16/821,644 is a continuation of application No. 16/564,375, filed on Sep. 9, 2019, granted, now 10,633,693, issued on Apr. 28, 2020.
Claims priority of provisional application 62/834,824, filed on Apr. 16, 2019.
Prior Publication US 2021/0164023 A1, Jun. 3, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6811 (2018.01); C12Q 1/6874 (2018.01); C12N 15/10 (2006.01)
CPC C12Q 1/6811 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6874 (2013.01); C12Q 2563/179 (2013.01); C12Q 2565/629 (2013.01); C12Q 2600/178 (2013.01)] 19 Claims
 
1. A method comprising:
receiving, in single-cell format, a set of target cells into an array of wells defined at a surface plane of a substrate;
receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles across the array of wells;
achieving a state of specific capture for the array of wells, wherein a well of the array of wells in the state of specific capture has a target cell of the set of target cells and a particle of the set of particles;
receiving a lysis buffer into a cavity over the array of wells;
receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer within the cavity and partitioning each of the array of wells from adjacent wells of the array of wells, at the surface plane;
lysing the set of target cells; and
retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells, thereby mitigating leakage of intracellular material of a first well to a second well of the array of wells.