	US 11,814,659 B2
	Tunable nanoscale cages from self-assembling DNA and protein building blocks
Nicholas Stephanopoulos, Scottsdale, AZ (US); and Yang Xu, Chandler, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed by Nicholas Stephanopoulos, Scottsdale, AZ (US); and Yang Xu, Chandler, AZ (US)
Filed on Mar. 11, 2020, as Appl. No. 16/816,029.
Claims priority of provisional application 62/816,729, filed on Mar. 11, 2019.
Prior Publication US 2020/0289658 A1, Sep. 17, 2020
Int. Cl. C12N 9/88 (2006.01); C07K 19/00 (2006.01); B82Y 5/00 (2011.01); C12N 15/11 (2006.01)

CPC C12N 9/88 (2013.01) [B82Y 5/00 (2013.01); C07K 19/00 (2013.01); C12N 15/11 (2013.01); C12Y 401/02014 (2013.01)]

6 Claims

1. A three-dimensional nanocage comprising:

a protein covalently linked to a polynucleotide handle, wherein the protein is a homotrimer;

wherein the polynucleotide handle is covalently linked to a solvent exposed cysteine by a disulfide linkage or

wherein the polynucleotide handle is covalently linked to a surface 4-azidophenylalanine residue; and

wherein the polynucleotide handle is at least 15 base pairs long; and

a DNA assembly comprising a polynucleotide arm complementary to the polynucleotide handle linked to the protein, wherein the polynucleotide handle and the polynucleotide arm form a double-stranded complex linked to the protein to the DNA assembly; and

wherein the DNA assembly comprises 4 oligonucleotides.