	US 11,814,638 B2
	*Marker associated with smut resistance in plant belonging to genus Saccharum* and use thereof**
Masaaki Mori, Okazaki (JP); Tatsuro Kimura, Kariya (JP); Hiroyuki Enoki, Hamamatsu (JP); Yusuke Ueta, Miyoshi (JP); Takeo Sakaigaichi, Miyakonojo (JP); Yusuke Tarumoto, Koshi (JP); Minoru Tanaka, Tsukuba (JP); Katsuki Adachi, Nishinoomote (JP); Taichiro Hattori, Nishinoomote (JP); Makoto Umeda, Nishinoomote (JP); and Michiko Hayano, Nishinoomote (JP)
Assigned to TOYOTA JIDOSHA KABUSHIKI KAISHA, Toyota (JP)
Appl. No. 17/257,453
Filed by TOYOTA JIDOSHA KABUSHIKI KAISHA, Toyota (JP)
PCT Filed Jul. 2, 2019, PCT No. PCT/JP2019/026324 § 371(c)(1), (2) Date Dec. 31, 2020, PCT Pub. No. WO2020/009113, PCT Pub. Date Jan. 9, 2020.
Claims priority of application No. 2018-127142 (JP), filed on Jul. 3, 2018; application No. 2018-197546 (JP), filed on Oct. 19, 2018; and application No. 2019-122913 (JP), filed on Jul. 1, 2019.
Prior Publication US 2021/0139929 A1, May 13, 2021
Int. Cl. C12Q 1/6895 (2018.01); A01H 1/04 (2006.01); A01H 1/00 (2006.01); C12N 15/82 (2006.01)

CPC C12N 15/8282 (2013.01) [A01H 1/045 (2021.01); A01H 1/1255 (2021.01); C12Q 1/6895 (2013.01); C12Q 2600/156 (2013.01)]

6 Claims

1. A method for producing a sugarcane line having improved smut resistance, the method comprising:

a step of breeding parent plants to obtain a progeny plant, wherein at least one of said parent plants is a sugarcane plant;

a step of extracting a genomic DNA of the progeny plant;

a step of determining the presence or absence of a marker associated with sugarcane smut resistance in the obtained genomic DNA;

a step of selecting a progeny plant having the marker associated with sugarcane smut resistance, and

a step of breeding only the selected progeny plant;

wherein the marker associated with sugarcane smut resistance comprises any nucleotide sequence selected from the group consisting of the nucleotide sequences of SEQ ID NOs:1 to 6, or at least 15 consecutive nucleotides of SEQ ID NOs:1 to 6.