US 11,814,621 B2
Expanding the chemical substrates for genetic code reprogramming
Michael Christopher Jewett, Evanston, IL (US); Joongoo Lee, Evanston, IL (US); Jeffrey S. Moore, Savoy, IL (US); Kenneth E. Schwieter, Champaign, IL (US); and Kevin Jerome Schwarz, Champaign, IL (US)
Assigned to Northwestern University, Evanston, IL (US); and The Board of Trustees of the University of Illinois, Urbana, IL (US)
Appl. No. 15/734,182
Filed by Northwestern University, Evanston, IL (US)
PCT Filed Jun. 3, 2019, PCT No. PCT/US2019/035215
§ 371(c)(1), (2) Date Dec. 1, 2020,
PCT Pub. No. WO2020/040840, PCT Pub. Date Feb. 27, 2020.
Claims priority of provisional application 62/679,350, filed on Jun. 1, 2018.
Prior Publication US 2021/0230586 A1, Jul. 29, 2021
Int. Cl. C12N 15/113 (2010.01); C12P 19/34 (2006.01); C12N 15/11 (2006.01)
CPC C12N 15/113 (2013.01) [C12N 15/11 (2013.01); C12P 19/34 (2013.01); C12N 2310/351 (2013.01)] 20 Claims
 
1. An acylated tRNA molecule having a formula defined as:

OG Complex Work Unit Chemistry
wherein:
tRNA is a transfer RNA linked via a 3′ terminal ribonucleotide;
R is selected from the group consisting of cycloalkyl substituted with amino; heterocycloalkyl; and alkylheterocycloalkyl; and
wherein R has a primary amine group or a secondary amine group in a gamma position or a delta position.