US 11,814,619 B2
Compositions, methods, and systems for single cell barcoding and sequencing
Eleen Yee Lam Shum, San Carlos, CA (US); Janice Hoiyi Lai, Mountain View, CA (US); Hei Mun Christina Fan, Palo Alto, CA (US); and Stephen P. A. Fodor, Palo Alto, CA (US)
Assigned to ENUMERIX, INC., Palo Alto, CA (US)
Filed by Enumerix, Inc., Palo Alto, CA (US)
Filed on Jun. 7, 2022, as Appl. No. 17/834,649.
Application 17/834,649 is a continuation of application No. PCT/US2022/032097, filed on Jun. 3, 2022.
Claims priority of provisional application 63/196,815, filed on Jun. 4, 2021.
Prior Publication US 2022/0389410 A1, Dec. 8, 2022
Int. Cl. C12N 1/15 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); C12N 15/10 (2006.01)
CPC C12N 15/1065 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6874 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method comprising:
generating a plurality of droplets within a collecting container at a rate of at least 50,000 droplets per minute, wherein the plurality of droplets is generated from an aqueous mixture comprising a set of single cells and a set of functionalized particles configured for a single cell assay;
wherein a functionalized particle of the set of functionalized particles comprises:
a body,
a first subset of single cell analyte capture molecules coupled to the body,
a second subset of releasable molecules coupled to the body, and
a third subset of hybridization molecules coupled to the body, which hybridization molecules are configured to hybridize with nucleic acid molecules of other functionalized particles of the set of functionalized particles.