CPC C12N 15/1065 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6874 (2013.01)] | 21 Claims |
1. A method comprising:
generating a plurality of droplets within a collecting container at a rate of at least 50,000 droplets per minute, wherein the plurality of droplets is generated from an aqueous mixture comprising a set of single cells and a set of functionalized particles configured for a single cell assay;
wherein a functionalized particle of the set of functionalized particles comprises:
a body,
a first subset of single cell analyte capture molecules coupled to the body,
a second subset of releasable molecules coupled to the body, and
a third subset of hybridization molecules coupled to the body, which hybridization molecules are configured to hybridize with nucleic acid molecules of other functionalized particles of the set of functionalized particles.
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