CPC C11B 1/10 (2013.01) [C10B 53/02 (2013.01); C10G 3/40 (2013.01); C10L 1/026 (2013.01); C10L 1/08 (2013.01); C11B 3/00 (2013.01); C11B 3/02 (2013.01); C11B 7/00 (2013.01); C11C 3/00 (2013.01); C11C 3/04 (2013.01); C11C 3/10 (2013.01); C11C 3/12 (2013.01); C10L 2200/0476 (2013.01); C10L 2270/026 (2013.01); C10L 2290/06 (2013.01); C10L 2290/26 (2013.01); C10L 2290/544 (2013.01); Y02E 50/10 (2013.01); Y02P 30/20 (2015.11)] | 18 Claims |
1. A process for producing extracted lipid, the process comprising
i) extracting lipid from plant leaf pieces expressing an exogenous Wrinkled 1 (WRI1) polypeptide and an exogenous diacylglycerol acyltransferase (DGAT), and having reduced triacylglycerol (TAG) lipase activity or expressing an exogenous Leafy Cotyledon 2 (LEC2) polypeptide or both reduced TAG lipase activity and expressing an exogenous LEC2 polypeptide, the leaf pieces having a surface area of at least 1 cm2, a total non-polar lipid content of between 5% and 30% by weight on a dry weight basis, and an increased total non-polar lipid content relative to a corresponding leaf piece which expresses the exogenous WRI1 polypeptide and the exogenous DGAT1 polypeptide but which does not have reduced TAG lipase activity and does not express the exogenous LEC2 polypeptide, and
ii) recovering the extracted lipid,
thereby producing the extracted lipid.
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