	US 11,814,420 B2
	Peptide deficient-MHC class I/chaperone compositions and methods
Nikolaos G. Sgourakis, Philadelphia, PA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Appl. No. 17/256,162
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Jul. 3, 2019, PCT No. PCT/US2019/040616 § 371(c)(1), (2) Date Dec. 24, 2020, PCT Pub. No. WO2020/010261, PCT Pub. Date Jan. 9, 2020.
Claims priority of provisional application 62/846,229, filed on May 10, 2019.
Claims priority of provisional application 62/694,824, filed on Jul. 6, 2018.
Prior Publication US 2021/0269503 A1, Sep. 2, 2021
Int. Cl. C07K 14/74 (2006.01); C40B 30/04 (2006.01); B01J 19/00 (2006.01)

CPC C07K 14/70539 (2013.01) [B01J 19/0046 (2013.01); C40B 30/04 (2013.01); B01J 2219/00725 (2013.01)]

19 Claims

1. A method of a making peptide deficient-MHC class I/chaperone complex, the method comprising:

a) incubating an MHC class I heavy chain, an MHC class I light chain and a placeholder peptide under conditions wherein the MHC class I heavy chain, the MHC class I light chain and the placeholder peptide form a placeholder peptide-MHC class I (p*MHC I) complex; and

b) contacting the placeholder peptide-MHC class I complex with a dipeptide and Tapasin Binding Protein Related (TAPBPR) chaperone, thereby displacing the placeholder peptide from the placeholder peptide-MHC complex and forming the peptide deficient-MHC class I/chaperone complex.