US 11,813,614 B2
Cryogenic systems
Brian Schryver, Redwood City, CA (US)
Assigned to BIOLIFE SOLUTIONS, INC., Bothell, WA (US)
Filed by BioLife Solutions, Inc., Bothell, WA (US)
Filed on Dec. 9, 2021, as Appl. No. 17/547,102.
Application 17/547,102 is a continuation of application No. 16/427,613, filed on May 31, 2019, granted, now 11,229,913.
Application 16/427,613 is a continuation of application No. 14/895,484, granted, now 10,307,761, issued on Jun. 4, 2019, previously published as PCT/US2014/040756, filed on Jun. 3, 2014.
Claims priority of provisional application 61/928,367, filed on Jan. 16, 2014.
Claims priority of provisional application 61/890,036, filed on Oct. 11, 2013.
Claims priority of provisional application 61/879,624, filed on Sep. 18, 2013.
Claims priority of provisional application 61/873,298, filed on Sep. 3, 2013.
Claims priority of provisional application 61/860,801, filed on Jul. 31, 2013.
Claims priority of provisional application 61/830,354, filed on Jun. 3, 2013.
Prior Publication US 2022/0323962 A1, Oct. 13, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 7/00 (2006.01); B01L 7/04 (2010.01); C12M 1/00 (2006.01); F25D 3/12 (2006.01)
CPC B01L 7/50 (2013.01) [B01L 7/04 (2013.01); C12M 45/22 (2013.01); F25D 3/125 (2013.01); B01L 2200/025 (2013.01); B01L 2200/143 (2013.01); B01L 2300/025 (2013.01); B01L 2300/0854 (2013.01); B01L 2300/1894 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A system for cryogenic processing comprising:
a container comprising a bottom, a first side, a second side, and a third side forming an interior chamber with an open top, the bottom having an interior floor surface, the first side having a first interior side surface, the second side having a second interior side surface, and the third side having a third interior side surface; and
a perforated retainer including a first perforated portion, a second perforated portion, and at least one peripheral structure extending from at least one of the first perforated portion and the second perforated portion, the perforated retainer configured to be positioned in the interior chamber such that the first perforated portion is adjacent the first interior side surface and the second perforated portion is adjacent the second interior side surface to divide the interior chamber into a sample-holding portion and a dry ice retention space, and such that the at least one peripheral structure is adjacent the interior floor surface in the dry ice retention space, the sample-holding portion being between the first perforated portion and the second perforated portion, the dry ice retention space positioned on a first peripheral side and a second peripheral side of the sample-holding portion, perforations of the first perforated portion and the second perforated portion configured to exhaust gas directly into the interior chamber,
wherein the system is configured to allow for access to the interior floor surface of the sample-holding portion while maintaining the sample-holding portion at an equilibrium condition at a temperature below an ambient temperature.