US 11,813,588 B2
Chromatographic material having improved pH stability, method for preparation thereof and uses thereof
Xiaodong Liu, Cupertino, CA (US); Xiao Cui, Sunnyvale, CA (US); and Xuefei Sun, San Jose, CA (US)
Assigned to Dionex Corporation, Sunnyvale, CA (US)
Filed by DIONEX CORPORATION, Sunnyvale, CA (US)
Filed on Jan. 11, 2021, as Appl. No. 17/146,447.
Application 17/146,447 is a division of application No. 15/543,809, abandoned, previously published as PCT/US2016/012998, filed on Jan. 12, 2016.
Claims priority of provisional application 62/103,869, filed on Jan. 15, 2015.
Prior Publication US 2021/0213420 A1, Jul. 15, 2021
Int. Cl. B01D 15/20 (2006.01); B01D 15/32 (2006.01); B01J 20/289 (2006.01); B01J 20/32 (2006.01); C07H 15/23 (2006.01); C07H 15/232 (2006.01); C07H 15/234 (2006.01); C07H 15/238 (2006.01); B01J 20/287 (2006.01); B01J 20/30 (2006.01); C07D 493/04 (2006.01); C07H 1/06 (2006.01); C07H 15/224 (2006.01); C08G 77/392 (2006.01)
CPC B01J 20/287 (2013.01) [B01D 15/20 (2013.01); B01D 15/325 (2013.01); B01J 20/289 (2013.01); B01J 20/3078 (2013.01); B01J 20/3092 (2013.01); B01J 20/3204 (2013.01); B01J 20/328 (2013.01); B01J 20/3217 (2013.01); B01J 20/3272 (2013.01); C07D 493/04 (2013.01); C07H 1/06 (2013.01); C07H 15/224 (2013.01); C07H 15/23 (2013.01); C07H 15/232 (2013.01); C07H 15/234 (2013.01); C07H 15/238 (2013.01); C08G 77/392 (2013.01); B01J 2220/44 (2013.01); B01J 2220/52 (2013.01); B01J 2220/54 (2013.01); B01J 2220/58 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method of separating a sample, the method comprising:
flowing a mobile phase containing the sample, the sample comprising one or more aminoglycoside antibiotics, through a chromatographic column to chromatographically separate the one or more aminoglycoside antibiotics from each other or from one or more components of the sample, wherein the column is packed with a chromatographic material, the chromatographic material comprising:
a silica substrate having a surface; and
a polymeric layer covalently bound to the surface,
the polymeric layer comprising polymer molecules, each polymer molecule being attached to the surface via multiple siloxane bonds and each polymer molecule being connected to one or more functionalizing compounds that each comprise a functional group, wherein the functional group comprises C14-C22 alkyl, each polymer molecule containing multiple first reactive groups, and each polymer molecule containing multiple silyl groups for forming the multiple siloxane bonds,
wherein the multiple silyl groups are distributed along a length of each of the polymer molecules, and
wherein the polymeric layer is formed by covalently attaching the polymer molecules to the surface of the substrate via the multiple silyl groups distributed along the length of each of said polymer molecules and reacting the first reactive groups of the attached polymer molecules with the one or more functionalizing compounds, wherein the one or more functionalizing compounds each comprise a second reactive group, wherein the second reactive group is reactive with the first reactive group, and
wherein the pH of the mobile phase is 1 or less.