US 11,813,315 B2
Fibronectin based scaffold domain proteins that bind to myostatin
Sharon Cload, Cambridge, MA (US); Linda Engle, Framingham, MA (US); Dasa Lipovsek, Cambridge, MA (US); Malavi Madireddi, West Windsor, NJ (US); Ginger Chao Rakestraw, Somerville, MA (US); Joanna Swain, Concord, MA (US); Wenjun Zhao, Montgomery, NJ (US); Hui Wei, Princeton Junction, NJ (US); Aaron P. Yamniuk, Lawrenceville, NJ (US); Vidhyashankar Ramamurthy, Bangalore (IN); Alexander T. Kozhich, Princeton, NJ (US); Martin J. Corbett, Mount Holly, NJ (US); and Stanley Richard Krystek, Jr., Ringoes, NJ (US)
Assigned to Bristol-Myers Squibb Company, Princeton, NJ (US)
Filed by BRISTOL-MYERS SQUIBB COMPANY, Princeton, NJ (US)
Filed on Feb. 14, 2019, as Appl. No. 16/276,156.
Application 16/276,156 is a division of application No. 15/282,277, filed on Sep. 30, 2016, granted, now 10,245,302.
Application 15/282,277 is a division of application No. 14/481,641, filed on Sep. 9, 2014, granted, now 9,493,546, issued on Nov. 15, 2016.
Application 14/481,641 is a continuation of application No. 14/025,307, filed on Sep. 12, 2013, granted, now 8,853,154, issued on Oct. 7, 2014.
Claims priority of provisional application 61/780,005, filed on Mar. 13, 2013.
Claims priority of provisional application 61/700,697, filed on Sep. 13, 2012.
Prior Publication US 2019/0307855 A1, Oct. 10, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/39 (2006.01); C07K 14/78 (2006.01); C07K 16/46 (2006.01); A61K 45/06 (2006.01); G01N 33/74 (2006.01); A61K 47/60 (2017.01); C07K 14/435 (2006.01); C07K 14/765 (2006.01); C07K 14/79 (2006.01); C07K 16/18 (2006.01); A61K 38/17 (2006.01); A61K 47/64 (2017.01); A61K 38/00 (2006.01)
CPC A61K 38/39 (2013.01) [A61K 38/17 (2013.01); A61K 38/1709 (2013.01); A61K 45/06 (2013.01); A61K 47/60 (2017.08); A61K 47/642 (2017.08); C07K 14/435 (2013.01); C07K 14/765 (2013.01); C07K 14/78 (2013.01); C07K 14/79 (2013.01); C07K 16/18 (2013.01); C07K 16/46 (2013.01); G01N 33/74 (2013.01); A61K 38/00 (2013.01); C07K 2319/30 (2013.01); C07K 2319/31 (2013.01)] 20 Claims
 
1. A method of producing a myostatin-binding polypeptide comprising culturing a cell comprising a nucleic acid encoding a polypeptide comprising a fibronectin type Ill tenth domain (10Fn3) which binds to myostatin and comprises AB, BC, CD, DE, EF, and FG loops, wherein at least one loop of the BC, DE, and FG loops of the 10Fn3 domain has 0, 1, 2, or 3 amino acid substitutions relative to the respective BC, DE, and FG loops of SEQ ID NOs: 34, 39, and 75 under conditions for expressing the polypeptide and purifying the polypeptide.