CPC A61K 38/39 (2013.01) [A61K 38/17 (2013.01); A61K 38/1709 (2013.01); A61K 45/06 (2013.01); A61K 47/60 (2017.08); A61K 47/642 (2017.08); C07K 14/435 (2013.01); C07K 14/765 (2013.01); C07K 14/78 (2013.01); C07K 14/79 (2013.01); C07K 16/18 (2013.01); C07K 16/46 (2013.01); G01N 33/74 (2013.01); A61K 38/00 (2013.01); C07K 2319/30 (2013.01); C07K 2319/31 (2013.01)] | 20 Claims |
1. A method of producing a myostatin-binding polypeptide comprising culturing a cell comprising a nucleic acid encoding a polypeptide comprising a fibronectin type Ill tenth domain (10Fn3) which binds to myostatin and comprises AB, BC, CD, DE, EF, and FG loops, wherein at least one loop of the BC, DE, and FG loops of the 10Fn3 domain has 0, 1, 2, or 3 amino acid substitutions relative to the respective BC, DE, and FG loops of SEQ ID NOs: 34, 39, and 75 under conditions for expressing the polypeptide and purifying the polypeptide.
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