	US 11,813,302 B2
	Brassica events LBFLFK and LBFDAU and methods for detection thereof
Toralf Senger, Research Triangle Park, NC (US); Laurent Marty, Heidelberg (DE); Irene Kunze, Gatersleben (DE); Dietrich Rein, Berlin (DE); Jörg Bauer, Research Triangle Park, NC (US); and Carl Andre, Research Triangle Park, NC (US)
Assigned to BASF PLANT SCIENCE COMPANY GMBH, Ludwigshafen (DE)
Filed by BASF PLANT SCIENCE COMPANY GMBH, Ludwigshafen (DE)
Filed on Jun. 14, 2021, as Appl. No. 17/346,522.
Application 17/346,522 is a continuation of application No. 15/526,443, granted, now 11,033,593, previously published as PCT/EP2015/076596, filed on Nov. 13, 2015.
Claims priority of provisional application 62/234,373, filed on Sep. 29, 2015.
Claims priority of provisional application 62/079,622, filed on Nov. 14, 2014.
Prior Publication US 2021/0308202 A1, Oct. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 36/31 (2006.01); C12Q 1/6895 (2018.01); C12N 15/82 (2006.01); C12N 9/02 (2006.01); C12N 9/00 (2006.01); A61K 31/202 (2006.01)

CPC A61K 36/31 (2013.01) [C12Q 1/6895 (2013.01); A61K 31/202 (2013.01); C12N 9/0071 (2013.01); C12N 9/93 (2013.01); C12N 15/8247 (2013.01); C12Y 114/19 (2013.01); C12Y 114/19003 (2013.01); C12Y 114/19006 (2013.01); C12Y 602/01003 (2013.01)]

6 Claims

1. A method of detecting the presence of DNA corresponding to Brassica event LBFLFK in a sample comprising DNA, the method comprising the steps of:

(a) contacting the sample with an LBFLFK Locus 1 primer pair that, when used in a nucleic acid amplification reaction with genomic DNA from Brassica event LBFLFK, produces a Locus 1 amplicon that is diagnostic for Brassica event LBFLFK;

(b) performing a nucleic acid amplification reaction, thereby producing the Locus 1 amplicon; and

(c) detecting the amplicon, wherein the amplicon comprises the LBFLFK Locus 1 junction region SEQ ID NO:4 or SEQ ID NO:5, or the complement thereof.