US 11,812,743 B2
Compositions and methods for scalable production and delivery of biologicals
Ameer Hamza Shakeel, Leesburg, VA (US); Zachery George Davis, Charlottesville, VA (US); Joseph Thomas Frank, Charlottesville, VA (US); Sepehr Zomorodi, Charlottesville, VA (US); and Payam Pourtaheri, Charlottesville, VA (US)
Assigned to AgroSpheres, Inc., Charlottesville, VA (US)
Appl. No. 16/649,857
Filed by AgroSpheres, Inc., Charlottesville, VA (US)
PCT Filed Sep. 25, 2018, PCT No. PCT/US2018/052690
§ 371(c)(1), (2) Date Mar. 23, 2020,
PCT Pub. No. WO2019/060903, PCT Pub. Date Mar. 28, 2019.
Claims priority of provisional application 62/562,723, filed on Sep. 25, 2017.
Claims priority of provisional application 62/666,981, filed on May 4, 2018.
Prior Publication US 2020/0267971 A1, Aug. 27, 2020
Int. Cl. C12N 15/74 (2006.01); A01N 25/28 (2006.01); A01N 63/20 (2020.01); A01N 63/50 (2020.01)
CPC A01N 25/28 (2013.01) [A01N 63/20 (2020.01); A01N 63/50 (2020.01); C12N 15/74 (2013.01)] 20 Claims
 
1. An agricultural formulation comprising:
an intact minicell comprising a biologically active compound within said minicell, wherein said biologically active compound comprises
a nucleic acid, wherein the nucleic acid targets a transcript encoding a polypeptide within a cell of a target,
wherein said target is a plant or a pest, and wherein the minicell is ribonuclease deficient.