CPC G16B 20/30 (2019.02) [C12N 15/63 (2013.01); C12N 15/635 (2013.01); C12N 15/70 (2013.01); C12N 15/79 (2013.01); G16B 20/00 (2019.02); G16B 30/00 (2019.02); G16B 30/20 (2019.02); G16B 35/00 (2019.02); G16B 35/10 (2019.02); G16C 20/60 (2019.02); G16B 20/50 (2019.02)] | 7 Claims |
1. A host cell comprising a heterologous genetic circuit comprising at least two orthogonal sequence-specific DNA binding polypeptides, wherein the genetic circuit is a combination of logic gates,
wherein the logic gates are combined by having a connection promoter that is an output promoter of an upstream gate and an input promoter of a downstream gate,
wherein a stem loop ribozyme spacer sequence is included after the connection promoter, and
wherein the stem loop ribozyme spacer sequence comprises any one of SEQ ID NOS:209-218.
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