US 11,810,646 B2
Synthetic biology tools
Chunbo Lou, San Francisco, CA (US); Tae Seok Moon, San Francisco, CA (US); Virgil Rhodius, El Sobrante, CA (US); Brynne Stanton, San Francisco, CA (US); Alvin Tasmir, San Francisco, CA (US); Karsten Temme, San Francisco, CA (US); and Chris Voigt, San Francisco, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Feb. 13, 2020, as Appl. No. 16/789,993.
Application 16/789,993 is a continuation of application No. 15/064,206, filed on Mar. 8, 2016, granted, now 10,607,716.
Application 15/064,206 is a continuation of application No. 13/489,205, filed on Jun. 5, 2012, abandoned.
Claims priority of provisional application 61/493,733, filed on Jun. 6, 2011.
Prior Publication US 2021/0005279 A1, Jan. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); G16B 20/30 (2019.01); G16B 20/00 (2019.01); G16B 30/00 (2019.01); G16B 35/00 (2019.01); G16C 20/60 (2019.01); C12N 15/63 (2006.01); G16B 35/10 (2019.01); G16B 30/20 (2019.01); C12N 15/70 (2006.01); C12N 15/79 (2006.01); G16B 20/50 (2019.01)
CPC G16B 20/30 (2019.02) [C12N 15/63 (2013.01); C12N 15/635 (2013.01); C12N 15/70 (2013.01); C12N 15/79 (2013.01); G16B 20/00 (2019.02); G16B 30/00 (2019.02); G16B 30/20 (2019.02); G16B 35/00 (2019.02); G16B 35/10 (2019.02); G16C 20/60 (2019.02); G16B 20/50 (2019.02)] 7 Claims
 
1. A host cell comprising a heterologous genetic circuit comprising at least two orthogonal sequence-specific DNA binding polypeptides, wherein the genetic circuit is a combination of logic gates,
wherein the logic gates are combined by having a connection promoter that is an output promoter of an upstream gate and an input promoter of a downstream gate,
wherein a stem loop ribozyme spacer sequence is included after the connection promoter, and
wherein the stem loop ribozyme spacer sequence comprises any one of SEQ ID NOS:209-218.