US 11,808,777 B2
Method of analyzing diluted biological sample component
Susumu Osawa, Yotsukaido (JP); Shinya Sugimoto, Tokyo (JP); and Isao Yonekubo, Tokyo (JP)
Assigned to Leisure, Inc., Chuo-ku Tokyo (JP)
Filed by Leisure, Inc., Tokyo (JP)
Filed on Apr. 28, 2020, as Appl. No. 16/860,656.
Application 16/860,656 is a division of application No. 15/328,977, granted, now 10,712,354, previously published as PCT/JP2015/069378, filed on Jul. 6, 2015.
Claims priority of application No. PCT/JP2014/069718 (WO), filed on Jul. 25, 2014.
Prior Publication US 2020/0319216 A1, Oct. 8, 2020
Int. Cl. G01N 33/96 (2006.01); G01N 33/49 (2006.01); C12Q 1/40 (2006.01)
CPC G01N 33/96 (2013.01) [C12Q 1/40 (2013.01); C12Y 302/01023 (2013.01); G01N 33/49 (2013.01); G01N 2333/938 (2013.01); G01N 2496/80 (2013.01)] 8 Claims
 
1. A method of analyzing a biological sample component, comprising:
(a) diluting a blood sample with a diluent buffer, wherein
a predetermined amount of an internal standard substance is added into the diluent buffer, and
the diluent buffer is free of the internal standard substance in a state that the internal standard substance is not added into the diluent buffer;
(b) measuring a concentration of the internal standard substance comprised in the diluent buffer in a state that the blood sample is not added to the diluent buffer;
(c) measuring a concentration of the internal standard substance comprised in the diluent buffer in a state that the blood sample is added to the diluent buffer;
(d) determining a dilution ratio of plasma or serum based on measured values of the concentration of the internal standard substance; and
(e) quantifying a biological component in the plasma or the serum in the blood sample based on the determined dilution ratio,
wherein the internal standard substance is selected from the group consisting of elements belonging to alkali metals and alkaline earth metals.