US 11,808,776 B2
Method of analyzing diluted biological sample component
Susumu Osawa, Yotsukaido (JP); Shinya Sugimoto, Tokyo (JP); and Isao Yonekubo, Tokyo (JP)
Assigned to Leisure, Inc., Tokyo (JP)
Filed by Leisure, Inc., Tokyo (JP)
Filed on Apr. 28, 2020, as Appl. No. 16/860,549.
Application 16/860,549 is a division of application No. 15/328,977, granted, now 10,712,354, previously published as PCT/JP2015/069378, filed on Jul. 6, 2015.
Claims priority of application No. PCT/JP2014/069718 (WO), filed on Jul. 25, 2014.
Prior Publication US 2020/0319215 A1, Oct. 8, 2020
Int. Cl. G01N 33/96 (2006.01); G01N 33/49 (2006.01); C12Q 1/40 (2006.01)
CPC G01N 33/96 (2013.01) [C12Q 1/40 (2013.01); C12Y 302/01023 (2013.01); G01N 33/49 (2013.01); G01N 2333/938 (2013.01); G01N 2496/80 (2013.01)] 7 Claims
 
1. A method of quantitative analysis of a component to be analyzed in a blood sample, the method comprising
(a) diluting the blood sample with a diluent buffer to quantitatively analyze the component to be analyzed in blood, wherein
a predetermined amount of an internal standard substance is added into the diluent buffer so that the internal standard substance has a predetermined concentration in the diluent buffer, and
the internal standard substance is an element belonging to alkali metals or alkaline earth metals, and
(b) measuring a concentration of the internal standard substance and a concentration of an external standard substance in the blood sample diluted with the diluent buffer, wherein
the external standard substance is a component homeostatically comprised at a predetermined concentration in the blood sample and is selected from the group consisting of sodium, chloride, albumin, and total protein,
the diluent buffer is free of the internal standard substance and the external standard substance in a state that the internal standard substance is not added into the diluent buffer,
(c) determining a dilution factor of the blood sample based on measured values of the concentration of the internal standard substance and the concentration of the external standard substance, and
(d) quantifying a biological component in the blood sample based on the determined dilution factor.