CPC G01N 33/6803 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6874 (2013.01); G01N 1/30 (2013.01); G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G01N 33/582 (2013.01); G01N 2021/6439 (2013.01)] | 26 Claims |
1. A method for preparing a biological sample for spatial proteomic analysis, the method comprising:
(a) providing a substrate comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises a spatial barcode and a capture domain;
(b) providing the biological sample on a second substrate;
(c) aligning the biological sample on the second substrate with the substrate comprising the plurality of capture probes, such that at least a portion of the biological sample is aligned with at least a portion of the substrate comprising the plurality of capture probes;
(d) contacting the biological sample with a protein blocking buffer; and
(e) contacting the biological sample with a plurality of analyte capture agents, wherein an analyte capture agent of the plurality of analyte capture agents comprises:
(i) an analyte binding moiety that binds to a protein from the biological sample;
(ii) an analyte binding moiety barcode; and
(iii) an analyte capture sequence, wherein the analyte capture sequence hybridizes to the capture domain of the capture probe.
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