US 11,808,767 B2
Methods, compositions and systems for microfluidic assays
Daniel T. Chiu, Seattle, WA (US); Mengxia Zhao, Seattle, WA (US); Wyatt Nelson, Seattle, WA (US); and Perry G. Schiro, Seattle, WA (US)
Assigned to UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION, Seattle, WA (US)
Filed by UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION, Seattle, WA (US)
Filed on Sep. 16, 2022, as Appl. No. 17/932,687.
Application 17/932,687 is a continuation of application No. 16/702,176, filed on Dec. 3, 2019, granted, now 11,480,575.
Application 16/702,176 is a continuation of application No. 14/903,012, granted, now 10,527,626, previously published as PCT/US2014/045094, filed on Jul. 1, 2014.
Claims priority of provisional application 61/894,788, filed on Oct. 23, 2013.
Claims priority of provisional application 61/843,252, filed on Jul. 5, 2013.
Prior Publication US 2023/0023536 A1, Jan. 26, 2023
Int. Cl. G01N 33/574 (2006.01); B01L 3/00 (2006.01); G01N 15/06 (2006.01); G01N 15/14 (2006.01); G01N 33/49 (2006.01); G01N 21/64 (2006.01); G01N 33/53 (2006.01); G01N 35/10 (2006.01); G01N 35/00 (2006.01); G01N 15/10 (2006.01)
CPC G01N 33/57492 (2013.01) [B01L 3/502761 (2013.01); B01L 3/561 (2013.01); B01L 3/567 (2013.01); G01N 15/0612 (2013.01); G01N 15/0656 (2013.01); G01N 15/1456 (2013.01); G01N 15/1484 (2013.01); G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G01N 33/49 (2013.01); G01N 33/5304 (2013.01); B01L 3/502738 (2013.01); B01L 3/502776 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0832 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/06 (2013.01); B01L 2400/0633 (2013.01); B01L 2400/086 (2013.01); G01N 15/0618 (2013.01); G01N 35/0098 (2013.01); G01N 2015/0681 (2013.01); G01N 2015/0693 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/149 (2013.01); G01N 2035/00237 (2013.01); G01N 2035/00356 (2013.01); G01N 2035/1034 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A system for detecting a particle in a fluid sample, the system comprising:
a microfluidic chip comprising:
at least one sample input channel;
at least one directional flow channel; and
at least two output channels, wherein the at least one directional flow channel intersects the at least one sample input channel;
an electro-actuated valve disposed on a device that is not part of the microfluidic chip, wherein the electro-actuated valve controls flow of a fluid by controlling the at least one directional flow channel;
at least one detector capable of detecting one or more analytes in an aliquot of the fluid sample;
a source of radiation;
a source of light; and
a controller operatively coupled to the electro-actuated valve, the at least one detector, the source of radiation, and the source of light, wherein the controller is programmed containing logic that when executed causes the system to perform operations including:
illuminating the aliquot with the source of radiation; detecting a first signal from a first tag with the at least one detector, wherein the first tag is connected to a first structure, and wherein the first structure is configured to bind to a first marker;
assigning a value to the aliquot based on the first signal; reducing, with light from the source of light, a level of the first signal of the first tag;
contacting the one or more analytes with a second structure configured to bind to a second marker, wherein the second structure is connected to a second tag; and
detecting the second tag.