US 11,808,761 B2
Method for measuring immunostimulatory response of immune cell, method for determining ability to form immune synapse in immune cell, and cell analyzer
Masatoshi Yanagida, Hyogo (JP); Keiko Miwa, Hyogo (JP); and Yuma Oka, Hyogo (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on Jul. 29, 2021, as Appl. No. 17/388,223.
Application 17/388,223 is a continuation of application No. 15/949,577, filed on Apr. 10, 2018, granted, now 11,125,740.
Claims priority of application No. 2017-078407 (JP), filed on Apr. 11, 2017.
Prior Publication US 2021/0356454 A1, Nov. 18, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/50 (2006.01); G01N 15/14 (2006.01); G01N 33/58 (2006.01); G01N 15/10 (2006.01)
CPC G01N 33/505 (2013.01) [G01N 15/147 (2013.01); G01N 15/1434 (2013.01); G01N 15/1475 (2013.01); G01N 33/5058 (2013.01); G01N 33/582 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1497 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for fluorescently labeling an immune cell, comprising:
(i) contacting a measurement target immune cell, an immunostimulator, and a substance different from the measurement target immune cell; and
(ii) separating the measurement target immune cell and the substance different from the measurement target immune cell, and labeling a molecule which is a component of an immune synapse of the measurement target immune cell with a fluorescent substance, whereby the measurement target immune cell to which the fluorescent substance is bound is prepared.
 
14. A method for fluorescently labeling an immune cell, comprising:
(i) contacting a measurement target immune cell, an immunostimulator, and a substance different from the measurement target immune cell; and
(ii) separating the measurement target immune cell and the substance different from the measurement target immune cell, and binding a capturing body to the immunostimulator which is bound to the measurement target immune cell, the capturing body specifically binding to the immunostimulator and comprising a fluorescent substance, whereby a molecule which is a component of an immune synapse of the measurement target immune cell is labeled with a fluorescent substance, and the measurement target immune cell to which the fluorescent substance is bound is prepared.