US 11,808,742 B2
Multi-dimensional liquid chromatography with second dimension having a variable flow rate
Konstantin Shoykhet, Karlsruhe (DE); and Stephan Buckenmaier, Ettlingen (DE)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
Filed on Aug. 26, 2021, as Appl. No. 17/446,096.
Claims priority of application No. 2013339 (GB), filed on Aug. 26, 2020.
Prior Publication US 2022/0065827 A1, Mar. 3, 2022
Int. Cl. G01N 30/46 (2006.01); G01N 30/14 (2006.01); G01N 30/32 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/46 (2013.01) [G01N 30/14 (2013.01); G01N 30/32 (2013.01); G01N 2030/027 (2013.01); G01N 2030/324 (2013.01); G01N 2030/326 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A multi-dimensional liquid chromatography system, comprising:
a first liquid chromatography system configured for providing a first chromatographic separation of a sample fluid comprised in a first mobile phase and to provide a first effluent comprising at least a portion of the separated sample fluid,
a second liquid chromatography system configured for providing a second chromatographic separation of at least a portion of the first effluent comprised in a second mobile phase, and
a control unit configured to operate the first liquid chromatography system by maintaining a first flow rate of the first mobile phase substantially constant during the first chromatographic separation, and to operate the second liquid chromatography system during the second chromatographic separation according to a control value different from a second flow rate of the second mobile phase.