CPC C12Q 1/686 (2013.01) [B01F 25/10 (2022.01); B01F 33/30 (2022.01); B01L 3/50273 (2013.01); B01L 3/502776 (2013.01); B01L 3/502784 (2013.01); B01L 7/525 (2013.01); G01N 35/08 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/141 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/185 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0487 (2013.01); G01N 35/1095 (2013.01); G01N 2035/00514 (2013.01)] | 19 Claims |
1. A system for analyzing a biological sample, the system comprising:
a sample preparation subsystem comprising:
a first fluid supply line configured to supply a flow of the biological sample containing target nucleic acid;
a second fluid supply line configured to supply a flow of carrier fluid immiscible with the biological sample, and
a chamber fluidically coupled to the first and second fluid supply lines,
wherein the sample preparation subsystem is configured to:
merge the flow of the biological sample from the first fluid supply line with the flow of carrier fluid from the second fluid supply line so as to separate strands of the target nucleic acid from the biological sample, thereby enabling manipulation of discrete volumes containing the separated strands, and
accumulate the strands of target nucleic acid enveloped in carrier fluid in the chamber;
a thermal cycling subsystem configured to receive, from the sample preparation subsystem, the accumulated strands of target nucleic acid enveloped in carrier fluid and to amplify the strands of target nucleic acid to generate amplified target nucleic acid; and
a detection subsystem configured to detect a signal from the amplified target nucleic acid.
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