US 11,807,898 B2
Method for determination of cellular mRNA
Mahmoud Labib, Toronto (CA); Edward H. Sargent, Toronto (CA); and Shana O. Kelley, Toronto (CA)
Assigned to The Governing Council of The University of Toronto, Toronto (CA)
Appl. No. 16/639,433
Filed by THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO, Toronto (CA)
PCT Filed Aug. 13, 2018, PCT No. PCT/CA2018/050977
§ 371(c)(1), (2) Date Feb. 14, 2020,
PCT Pub. No. WO2019/033203, PCT Pub. Date Feb. 21, 2019.
Claims priority of provisional application 62/545,081, filed on Aug. 14, 2017.
Prior Publication US 2021/0017585 A1, Jan. 21, 2021
Int. Cl. C12Q 1/6816 (2018.01); C12Q 1/6886 (2018.01)
CPC C12Q 1/6816 (2013.01) [C12Q 1/6886 (2013.01); C12Q 2563/143 (2013.01); C12Q 2563/155 (2013.01); C12Q 2565/629 (2013.01); C12Q 2600/158 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method of magnetically capturing cells, the method comprising:
introducing a first capture probe and a second capture probe into the cells, the first capture probe and the second capture probe each configured to be complementary to a respective section of target mRNA within the cells, wherein binding of the first and second capture probes to the respective sections of the target mRNA results in tagging of the cells and causes the first and second capture probes to form clusters with each other;
wherein the first capture probe is complementary to a first section of the target mRNA and the second capture probe is complementary to a second section of the target mRNA, wherein the first section of the target mRNA is different than the second section of the target mRNA;
wherein the first capture probe and the second capture probe are each bound to magnetic nanoparticles (MNPs) that, when trapped within tagged cells, cause the tagged cells to be susceptible to magnetic forces; and
introducing a plurality of cells comprising the tagged cells into a device configured to magnetically capture the tagged cells.
 
7. A system for analyzing mRNA in cells comprising:
a first capture probe and a second capture probe, the first capture probe and the second capture probe each configured to be complementary to a respective section of target mRNA within the cells, wherein binding of the first and second capture probes to the respective sections of the target mRNA results in tagging of the cells and causes the first and second capture probes to form clusters with each other;
wherein the first capture probe is complementary to a first section of the target mRNA and the second capture probe is complementary to a second section of the target mRNA, wherein the first section of the target mRNA is different than the second section of the target mRNA;
wherein the first capture probe and the second capture probe are each bound to magnetic nanoparticles (MNPs) that, when trapped within the tagged cells, cause the tagged cells to be susceptible to magnetic forces; and
a device configured to magnetically capture tagged cells.