US 11,807,889 B2
Yeast expressing a heterologous phospholipase for ethanol production
Monica Tassone, West Sacramento, CA (US); James Ron Huffman, Wake Forest, NC (US); Chee-Leong Soong, Raleigh, NC (US); and Jung Yi, Sacramento, CA (US)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Appl. No. 17/283,760
Filed by Novozymes A/S, Bagsvaerd (DK)
PCT Filed Oct. 7, 2019, PCT No. PCT/US2019/054996
§ 371(c)(1), (2) Date Apr. 8, 2021,
PCT Pub. No. WO2020/076697, PCT Pub. Date Apr. 16, 2020.
Claims priority of provisional application 62/742,805, filed on Oct. 8, 2018.
Prior Publication US 2021/0381009 A1, Dec. 9, 2021
Int. Cl. C12P 7/06 (2006.01); C12N 9/16 (2006.01); C12N 9/18 (2006.01); C12N 9/34 (2006.01)
CPC C12P 7/06 (2013.01) [C12N 9/16 (2013.01); C12N 9/18 (2013.01); C12N 9/2428 (2013.01); C12Y 301/01004 (2013.01); C12Y 301/04003 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01)] 20 Claims
 
1. A recombinant Saccharomyces yeast cell comprising a heterologous polynucleotide encoding a phospholipase and a heterologous polynucleotide encoding a glucoamylase,
wherein the phospholipase has a mature polypeptide sequence at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 237.