	US 11,807,879 B2
	Compositions of beta-glucuronidase enzyme blends with enhanced enzymatic activity and methods of preparation thereof
John Tomashek, Columbia, SC (US); Caleb Reece Schlachter, Irmo, SC (US); Pongkwan Sitasuwan, Columbia, SC (US); and Lim Andrew Lee, Columbia, SC (US)
Assigned to INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC., Irmo, SC (US)
Filed by INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC., Irmo, SC (US)
Filed on Dec. 10, 2021, as Appl. No. 17/547,854.
Application 17/547,854 is a division of application No. 16/528,292, filed on Jul. 31, 2019, granted, now 11,268,079.
Claims priority of provisional application 62/754,358, filed on Nov. 1, 2018.
Claims priority of provisional application 62/713,188, filed on Aug. 1, 2018.
Prior Publication US 2022/0090037 A1, Mar. 24, 2022
Int. Cl. C12N 9/24 (2006.01); C12Q 1/37 (2006.01); C12Q 1/00 (2006.01)

CPC C12N 9/2405 (2013.01) [C12Q 1/005 (2013.01); C12Q 1/37 (2013.01); C12Y 301/06001 (2013.01); C12Y 302/01031 (2013.01)]

12 Claims

1. A composition comprising a blend of Eubacterium eligens beta-glucuronidase (EeGUS) enzyme and a chimeric beta-glucuronidase enzyme Rxn3, wherein EeGUS comprises the amino acid sequence shown in SEQ ID NO: 10 and Rxn3 comprises the amino acid sequence shown in SEQ II) NO: 19.