US 11,807,859 B2
Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
Ralph E. Dewey, Apex, NC (US); Balazs Siminszky, Neuchatel (CH); Steven W. Bowen, Raleigh, NC (US); and Lily Gavilano, Raleigh, NC (US)
Assigned to North Carolina State University, Raleigh, NC (US); and University of Kentucky Research Foundation, Lexington, KY (US)
Filed by North Carolina State University, Raleigh, NC (US); and University of Kentucky Research Foundation, Lexington, KY (US)
Filed on Mar. 1, 2022, as Appl. No. 17/653,059.
Application 13/361,159 is a division of application No. 12/269,531, filed on Nov. 12, 2008, granted, now 8,124,851, issued on Feb. 28, 2012.
Application 13/361,235 is a division of application No. 12/269,531, filed on Nov. 12, 2008, granted, now 8,124,851, issued on Feb. 28, 2012.
Application 17/653,059 is a continuation of application No. 16/860,542, filed on Apr. 28, 2020, granted, now 11,293,031.
Application 16/860,542 is a continuation of application No. 15/631,873, filed on Jun. 23, 2017, granted, now 10,687,490, issued on Jun. 23, 2020.
Application 15/631,873 is a continuation of application No. 14/950,155, filed on Nov. 24, 2015, granted, now 10,292,353, issued on May 21, 2019.
Application 14/950,155 is a continuation of application No. 13/361,235, filed on Jan. 30, 2012, granted, now 9,228,195, issued on Jan. 5, 2016.
Application 13/361,235 is a continuation of application No. 13/361,159, filed on Jan. 30, 2012, granted, now 9,228,194, issued on Jan. 5, 2016.
Claims priority of provisional application 60/987,243, filed on Nov. 12, 2007.
Prior Publication US 2022/0275388 A1, Sep. 1, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A01H 1/06 (2006.01); C12N 9/02 (2006.01); C12Q 1/6895 (2018.01)
CPC C12N 15/8243 (2013.01) [A01H 1/06 (2013.01); C12N 9/0073 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)] 21 Claims
 
1. A tobacco seed, a tobacco plant grown therefrom, or a part of said tobacco plant, wherein said tobacco seed, tobacco plant grown therefrom, or part of said tobacco plant comprises a variant polynucleotide comprising a non-natural mutation as compared to a reference sequence, wherein said reference sequence comprises at least 95% sequence identity compared to SEQ ID NO:4, and wherein said non-natural mutation is positioned between two sequences corresponding to SEQ ID NOs: 36 and 37.