	US 11,807,668 B2
	Use of citronellol in preparing preparation for promoting expression of virulence gene Toxa of Pseudomonas aeruginosa
Wenru Li, Guangzhou (CN); Xiaobao Xie, Guangzhou (CN); Taohua Zeng, Guangzhou (CN); Zhiqing Zhang, Guangzhou (CN); Jingxia Liu, Guangzhou (CN); and Qingshan Shi, Guangzhou (CN)
Assigned to INSTITUTE OF MICROBIOLOGY, GUANGDONG ACADEMY OF SCIENCES (GUANGDONG DETECTION CENTER OF MICRORI, Guangzhou (CN)
Appl. No. 17/778,018
Filed by Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Detection Center of Microbiology), Guangzhou (CN)
PCT Filed Oct. 27, 2021, PCT No. PCT/CN2021/126812 § 371(c)(1), (2) Date May 19, 2022, PCT Pub. No. WO2022/068954, PCT Pub. Date Apr. 7, 2022.
Claims priority of application No. 202110435173.6 (CN), filed on Apr. 22, 2021.
Prior Publication US 2023/0010612 A1, Jan. 12, 2023
Int. Cl. C07K 14/21 (2006.01); C12N 1/20 (2006.01)

CPC C07K 14/21 (2013.01) [C12N 1/20 (2013.01)]

5 Claims

1. A method of using citronellol to promote expression of a virulence gene toxA in Pseudomonas aeruginosa, comprising:

preparing a liquid LB culture medium with citronellol, wherein the concentration of the citronellol in the culture medium is 1.25 μL/mL;

inoculating the medium with a bacterial suspension of Pseudomonas aeruginosa in the exponential growth phase, wherein the expression of the virulence gene toxA results in production of an exotoxin A in the Pseudomonas aeruginosa;

up-regulating expression of a signaling molecule synthase gene lasI and a signaling molecule receptor protein gene lasR of a las system;

up-regulating expression of a signaling molecule receptor protein gene rhIR in a rhI system;

inhibiting expression of a signaling molecule synthase gene pqsA and a signaling molecule receptor protein gene pqsR of a pqs system; and

inhibiting expression of virulence genes lasB, phzM, chiC and psIB.