	US 11,807,666 B2
	Chromatographic methods for purification of proteins from plasma
Martin Hall, Uppsala (SE); Karolina Busson, Uppsala (SE); Jean-Luc Maloisel, Uppsala (SE); and Helena Skoglar, Uppsala (SE)
Assigned to Cytiva BioProcess R&D AB, Uppsala (SE)
Filed by Cytiva BioProcess R&D AB, Uppsala (SE)
Filed on Nov. 29, 2022, as Appl. No. 18/059,929.
Application 18/059,929 is a continuation of application No. 15/564,232, granted, now 11,542,300, previously published as PCT/EP2016/057356, filed on Apr. 4, 2016.
Claims priority of application No. 1506117 (GB), filed on Apr. 10, 2015.
Prior Publication US 2023/0091082 A1, Mar. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/18 (2006.01); B01J 41/20 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); A61K 38/36 (2006.01); A61K 38/48 (2006.01); A61K 35/16 (2015.01); C12N 9/64 (2006.01); B01D 15/34 (2006.01); C07K 14/47 (2006.01); C07K 14/755 (2006.01); C07K 14/765 (2006.01); C07K 16/00 (2006.01); A61K 38/00 (2006.01)

CPC C07K 1/18 (2013.01) [A61K 35/16 (2013.01); A61K 38/36 (2013.01); A61K 38/4846 (2013.01); B01D 15/34 (2013.01); B01D 15/363 (2013.01); B01D 15/3847 (2013.01); B01J 41/20 (2013.01); C07K 14/472 (2013.01); C07K 14/755 (2013.01); C07K 14/765 (2013.01); C07K 16/00 (2013.01); C12N 9/644 (2013.01); C12N 9/6424 (2013.01); C12Y 304/21022 (2013.01); A61K 38/00 (2013.01)]

16 Claims

1. A chromatographic method comprising the following steps:

loading proteins on a chromatography column packed with a resin comprising porous lid beads having an inner porous core and an outer porous lid, wherein the inner core is provided with anion exchange ligands, and wherein the porosity of the lid and core does not allow entering of molecules larger than 500 kD;

adsorbing Factor IX (FIX) on the anion exchange ligands in the core;

collecting separated proteins in the flow through; and

eluting FIX from the ligands in the core.