US 11,807,665 B2
Detection and quantification of glycosylated peptides
Avraham Z. Rosenberg, Passaic, NJ (US); and Biao Shen, Dobbs Ferry, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Jul. 12, 2019, as Appl. No. 16/509,798.
Claims priority of provisional application 62/697,547, filed on Jul. 13, 2018.
Prior Publication US 2020/0017544 A1, Jan. 16, 2020
Int. Cl. C07K 1/16 (2006.01); B01D 15/30 (2006.01); B01D 15/42 (2006.01); G01N 33/68 (2006.01)
CPC C07K 1/16 (2013.01) [B01D 15/305 (2013.01); B01D 15/426 (2013.01); G01N 33/6848 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of separating glycopeptides, comprising:
contacting a sample comprising glycopeptides to a hydrophilic enrichment substrate under conditions that permit the glycopeptides to bind to the hydrophilic enrichment substrate;
washing the hydrophilic enrichment substrate to remove non-glycopeptide contaminants from the hydrophilic enrichment substrate;
eluting the glycopeptides from the hydrophilic enrichment substrate with an ammonium formate and acetonitrile (ACN) in water solution to create an enriched glycopeptide sample;
applying the enriched glycopeptide sample to a separation column; and
eluting the glycopeptides from the separation column, thereby separating glycopeptides in the sample.