CPC C07K 1/16 (2013.01) [B01D 15/305 (2013.01); B01D 15/426 (2013.01); G01N 33/6848 (2013.01)] | 20 Claims |
1. A method of separating glycopeptides, comprising:
contacting a sample comprising glycopeptides to a hydrophilic enrichment substrate under conditions that permit the glycopeptides to bind to the hydrophilic enrichment substrate;
washing the hydrophilic enrichment substrate to remove non-glycopeptide contaminants from the hydrophilic enrichment substrate;
eluting the glycopeptides from the hydrophilic enrichment substrate with an ammonium formate and acetonitrile (ACN) in water solution to create an enriched glycopeptide sample;
applying the enriched glycopeptide sample to a separation column; and
eluting the glycopeptides from the separation column, thereby separating glycopeptides in the sample.
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