US 11,806,714 B2
Selective delivery of material to cells
Armon R. Sharei, Cambridge, MA (US); Viktor A. Adalsteinsson, Cambridge, MA (US); Nahyun Cho, Closter, NJ (US); Robert S. Langer, Newton, MA (US); J. Christopher Love, Somerville, MA (US); and Klavs F. Jensen, Lexington, MA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Oct. 20, 2020, as Appl. No. 17/075,116.
Application 17/075,116 is a continuation of application No. 16/145,865, filed on Sep. 28, 2018, granted, now 10,870,112.
Application 16/145,865 is a continuation of application No. 14/912,001, granted, now 10,124,336, previously published as PCT/US2014/051343, filed on Aug. 15, 2014.
Claims priority of provisional application 61/866,972, filed on Aug. 16, 2013.
Prior Publication US 2021/0170411 A1, Jun. 10, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); C12N 5/09 (2010.01); C12N 5/078 (2010.01); G01N 15/14 (2006.01); C12Q 1/04 (2006.01); G01N 33/574 (2006.01); G01N 1/30 (2006.01); G01N 15/10 (2006.01); B82Y 30/00 (2011.01); C12M 3/06 (2006.01); G01N 15/00 (2006.01)
CPC B01L 3/502761 (2013.01) [C12N 5/0634 (2013.01); C12N 5/0693 (2013.01); C12Q 1/04 (2013.01); G01N 15/1459 (2013.01); G01N 15/1484 (2013.01); G01N 33/574 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/08 (2013.01); B01L 2400/0487 (2013.01); B82Y 30/00 (2013.01); C12M 23/16 (2013.01); G01N 1/30 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1081 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method for delivering a compound into a cell based on a physical property of the cell comprising:
providing a cell suspension comprising a first group of cells and a second group of cells, wherein the first group of cells have a relatively different size, diameter, and/or membrane stiffness than the second group of cells;
passing the cell suspension through and out of a microfluidic channel that includes a constriction, wherein, as the cell suspension passes through the microfluidic channel, the constriction induces greater disruption to a cell membrane of the first group of cells as compared to a cell membrane of the second group of cells, such that a greater amount of the compound enters the first group of cells through the disruptions as compared to the second group of cells when the first group of cells and the second group of cells are contacted with the compound; and
contacting the cell suspension with the compound.